重組小鼠白介素-4(rmIL-4)
- 型號(hào):
- 更新時(shí)間: 2024-05-23
- 上一個(gè):重組大鼠白介素-4(rrIL-4)
- 下一個(gè):重組人白介素-5 (rHuIL-5)
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產(chǎn)品介紹
CYT-282 | 重組小鼠白介素-4(rmIL-4) | 2μg/10μg/1mg | Prospec |
Description : | |
Recombinant Mouse IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13500 Dalton. Recombinant IL-4 is purified by proprietary chromatographic techniques. | |
Physical Appearance: | |
Sterile Filtered White lyophilized (freeze-dried) powder. | |
Formulation: | |
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. | |
Solubility: | |
It is recommended to reconstitute the lyophilized IL-4 in sterile 18Mω-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
Stability: | |
Lyophilized IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IL-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | |
Purity: | |
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
Amino acid sequence: | |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ile-His-Gly. | |
Dimers and aggregates: | |
Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
Biological Activity: | |
ProSpec's Interleukin-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant prolifiration of Murine HT-2 cells is less then 2 ng/ml, corresponding to a Specific Activity of 5x105 IU/mg. | |
Endotoxin: | |
Less than 0.1 ng/µg (IEU/µg) of IL-4. | |
Protein content: | |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard. | |
Usage: | |
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
佰易聚生物商城提供美國(guó)光譜醫(yī)學(xué)透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優(yōu)質(zhì)的分子生物學(xué)試劑和試劑盒、培養(yǎng)基本和培養(yǎng)耗材、細(xì)胞因子和細(xì)胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購(gòu)!
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