重組人酸性成纖維細胞生長因子（rHuFGF-a/gly）
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- 更新時間： 2025-02-12
- 上一個：重組人酸性成纖維細胞生長因子（rHuFGF-a）
- 下一個：重組人堿性成纖維細胞生長因子（rHuFGF-b/gly）
| CYT-364 | 重組人酸性成纖維細胞生長因子（rHuFGF-a/gly） | 2μg/10μg/1mg | Prospec |
| Background : | |
Single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. It binds to heparin, which potentiates its biological activity and protects it from proteolysis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages, and also has chemotactic and mitogenic activities. It was originally named acidic fibroblast growth factor based upon its chemical properties and to distinguish it from basic fibroblast growth factor. FGF-6 , K-FGF and KGF （keratinocyte growth factor =FGF-7 ）. All factors are products of different genes, some of which are Oncogene products （FGF-3 , FGF-4 , FGF-5 ）. | |
 | Description : |
Recombinant Human FGF-a （FGF-1） produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of 15803 Dalton. techniques. | |
| Physical Appearance: |
| Sterile Filtered liquid formulation. | |
| Formulation: |
The sterile protein solution （1.8mg/ml） contains 20mM Tris HCl pH=7.9, 100mM KCl, 0.2mM DTT and 20% glycerol. | |
| Stability: |
Recombinant Human FGF-a although stable at 40C for 3 weeks, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. | |
| Purity: |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Asn-Leu-Pro. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
ProSpec’s Recombinant Human FGF-a is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors （measured by 3H-thymidine uptake） is <10 ng/ml, corresponding to a specific activity of 105 Units/mg. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of FGF-acidic-Sf9. | |
| Protein content: |
Protein quantitation was carried out by two independent methods: Standard. | |
| Usage: |
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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